psq™hs 96a pyrosequencing system (BIOTAGE)
90
Structured Review
BIOTAGE
psq™hs 96a pyrosequencing system
Psq™Hs 96a Pyrosequencing System, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psq+hs+96a+system/psq+hs+96a+system/pmc02753619-115-23-27
Average 90 stars, based on 1 article reviews
Psq™Hs 96a Pyrosequencing System, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/psq+hs+96a+system/psq+hs+96a+system/pmc02753619-115-23-27
Average 90 stars, based on 1 article reviews
psq™hs 96a pyrosequencing system - by Bioz Stars,
2026-09
90/100 stars
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Polymerase Chain Reaction:Article Title: Methylation of the CDX2 promoter in Helicobacter pylori-infected gastric mucosa increases with age and its rapid demethylation in gastric tumors is associated with upregulated gene expression. Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in 20 μl reactions using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 347-bp product using the primer set shown in Supplementary Table 1 (available at Carcinogenesis Online). .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 45 cycles of 94°C for 30 s, 54°C for 40 s and 72°C for 30 s, with a final incubation at 72°C for 5 min. Pyrosequencing was performed as described (33) using the sequencing primer in Supplementary Table 1 (available at Carcinogenesis Online)and the Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model. Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221‐bp product using the following primers: Forward, 5'‐TGT TTG GGG GTA GGT AGT AGT TAG‐3 and reverse, 5'‐CCC TAA ACC CAC ATA CTA AAC TTT C‐3'. .. PCR was performed using an initial melting step of 94 ̊C for 5 min, followed by 40 cycles of 94 ̊C for 30 sec, 59 ̊C for 30 sec and 72 ̊C for 30 sec, with a final incubation at 72 ̊C for 7 min. Pyrosequencing was performed as previously described (17) using a sequencing primer (5'‐AGG AGT AGT GTG GGG TTT‐3') and Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221-bp product using the following primers: Forward, 5′-TGTTTGGGGGTAGGTAGTAGTTAG-3 and reverse, 5′-CCCTAAACCCACATACTAAACTTTC-3′. .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as previously described ( ) using a sequencing primer (5′-AGGAGTAGTGTGGGGTTT-3′) and Article Title: ONECUT2 upregulation is associated with CpG hypomethylation at promoter‐proximal DNA in gastric cancer and triggers ACSL5 Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20‐μl reaction using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 199‐bp product using the primer set shown in Supporting Information Table . .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as described using the sequencing primer in Supporting Information Table and the Incubation:Article Title: Methylation of the CDX2 promoter in Helicobacter pylori-infected gastric mucosa increases with age and its rapid demethylation in gastric tumors is associated with upregulated gene expression. Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in 20 μl reactions using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 347-bp product using the primer set shown in Supplementary Table 1 (available at Carcinogenesis Online). .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 45 cycles of 94°C for 30 s, 54°C for 40 s and 72°C for 30 s, with a final incubation at 72°C for 5 min. Pyrosequencing was performed as described (33) using the sequencing primer in Supplementary Table 1 (available at Carcinogenesis Online)and the Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model. Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221‐bp product using the following primers: Forward, 5'‐TGT TTG GGG GTA GGT AGT AGT TAG‐3 and reverse, 5'‐CCC TAA ACC CAC ATA CTA AAC TTT C‐3'. .. PCR was performed using an initial melting step of 94 ̊C for 5 min, followed by 40 cycles of 94 ̊C for 30 sec, 59 ̊C for 30 sec and 72 ̊C for 30 sec, with a final incubation at 72 ̊C for 7 min. Pyrosequencing was performed as previously described (17) using a sequencing primer (5'‐AGG AGT AGT GTG GGG TTT‐3') and Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221-bp product using the following primers: Forward, 5′-TGTTTGGGGGTAGGTAGTAGTTAG-3 and reverse, 5′-CCCTAAACCCACATACTAAACTTTC-3′. .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as previously described ( ) using a sequencing primer (5′-AGGAGTAGTGTGGGGTTT-3′) and Article Title: ONECUT2 upregulation is associated with CpG hypomethylation at promoter‐proximal DNA in gastric cancer and triggers ACSL5 Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20‐μl reaction using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 199‐bp product using the primer set shown in Supporting Information Table . .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as described using the sequencing primer in Supporting Information Table and the Sequencing:Article Title: Methylation of the CDX2 promoter in Helicobacter pylori-infected gastric mucosa increases with age and its rapid demethylation in gastric tumors is associated with upregulated gene expression. Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in 20 μl reactions using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 347-bp product using the primer set shown in Supplementary Table 1 (available at Carcinogenesis Online). .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 45 cycles of 94°C for 30 s, 54°C for 40 s and 72°C for 30 s, with a final incubation at 72°C for 5 min. Pyrosequencing was performed as described (33) using the sequencing primer in Supplementary Table 1 (available at Carcinogenesis Online)and the Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model. Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221‐bp product using the following primers: Forward, 5'‐TGT TTG GGG GTA GGT AGT AGT TAG‐3 and reverse, 5'‐CCC TAA ACC CAC ATA CTA AAC TTT C‐3'. .. PCR was performed using an initial melting step of 94 ̊C for 5 min, followed by 40 cycles of 94 ̊C for 30 sec, 59 ̊C for 30 sec and 72 ̊C for 30 sec, with a final incubation at 72 ̊C for 7 min. Pyrosequencing was performed as previously described (17) using a sequencing primer (5'‐AGG AGT AGT GTG GGG TTT‐3') and Article Title: Differential expression and methylation of integrin subunit alpha 11 and thrombospondin in the amnion of preterm birth Article Snippet: .. Pyrosequencing reactions for ITGA11 and THBS2 gene methylation were conducted with sequencing primers on the Article Title: STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model Article Snippet: Bisulfite-modified DNA (100 ng) was amplified by PCR in a 20 μl reaction using 2X Dye Mix polymerase (Enzynomics Co., Ltd.) to yield a 221-bp product using the following primers: Forward, 5′-TGTTTGGGGGTAGGTAGTAGTTAG-3 and reverse, 5′-CCCTAAACCCACATACTAAACTTTC-3′. .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as previously described ( ) using a sequencing primer (5′-AGGAGTAGTGTGGGGTTT-3′) and Article Title: ONECUT2 upregulation is associated with CpG hypomethylation at promoter‐proximal DNA in gastric cancer and triggers ACSL5 Article Snippet: Bisulfite‐modified DNA (100 ng) was amplified by PCR in a 20‐μl reaction using 2× Dye Mix polymerase (Enzynomics, Daejeon, Korea) to yield a 199‐bp product using the primer set shown in Supporting Information Table . .. PCR was performed using an initial melting step of 94°C for 5 min, followed by 40 cycles of 94°C for 30 sec, 59°C for 30 sec and 72°C for 30 sec, with a final incubation at 72°C for 7 min. Pyrosequencing was performed as described using the sequencing primer in Supporting Information Table and the DNA Methylation Assay:Article Title: Differential DNA methylation of MSI2 and its correlation with diabetic traits Article Snippet: .. Pyrosequencing assays were designed, optimized, and performed on the Methylation:Article Title: Differential expression and methylation of integrin subunit alpha 11 and thrombospondin in the amnion of preterm birth Article Snippet: .. Pyrosequencing reactions for ITGA11 and THBS2 gene methylation were conducted with sequencing primers on the |
